Fermentation tank refers to a device used in industry for microbial fermentation. Its main body is generally a main cylinder made of stainless steel plate, and its volume is from 1ml to several hundred ml. In the design and processing, attention should be paid to the strict and reasonable structure. Can withstand steam sterilization, have certain operating flexibility, minimize internal accessories (avoid dead ends), strong material and energy transfer performance, and can be adjusted to facilitate cleaning, reduce pollution, suitable for the production of various products and reduce energy consume.
Fermentation tanks are divided into mechanical stirring and ventilation fermentation tanks and non-mechanical stirring ventilation fermentation tanks; according to the growth and metabolism needs of microorganisms, they are divided into aerobic fermentation tanks and anaerobic fermentation tanks.
Laboratory fermenter is a kind of equipment for mechanical stirring and fermentation of materials. The equipment adopts internal circulation mode, uses stirring paddle to disperse and break bubbles, it has high oxygen dissolution rate and good mixing effect. When the fermentation tank is finished, clean the tank body and electrodes, insert the pH electrode into the triangular flask with 3M potassium chloride for use, and cover the probe of the dissolved oxygen electrode with a protective sleeve and save it for future use.
In use, some microorganisms have higher requirements for sterilization, such as food companies, etc., the fermentation tank needs to be sterilized with high pressure steam, such as antibiotic production; some are lower, and hot water sterilization is sufficient, such as alcohol fermentation.
The structure of the biological fermentation tank is relatively complex, and it is not easy to sterilize the internal edges and corners. The general fermentation tank has an automatic cleaning device, which is thoroughly sterilized after cleaning.
1. Before sterilizing the medium, the sub-air filter connected to the tank should usually be sterilized with steam and dried with air. When the solid tank is sterilized, the sewage in the conveying pipeline is firstly drained and rinsed, and then the prepared culture medium is pumped into the fermentation tank (seed tank or material tank), and the agitator is started at the same time for sterilization. No matter how the pipeline connected to the tank is prepared, the principle of “no entry or exit” should be followed during disinfection. This can ensure complete sterilization without leaving dead ends. During sterilization, the total steam pipeline pressure shall not be lower than 0.3~0.35Mpa, and the operating pressure shall not be lower than 0.2Mpa.
2. Empty tank sterilization (empty elimination) is the sterilization of the fermenter tank. When emptying, the tank pressure is generally maintained at 0.15~0.2Mpa, and the tank temperature is 125~130℃ for 30~45min; the total steam pressure is required to be no less than 0.3~0.35Mpa, and the steam pressure used is no less than 0.25~0.3MPa. The disinfection of biological fermentation tanks is a part that cannot be ignored in production. It must be carefully fermented. The pharmaceutical and food industry pays attention to the disinfection of fermentation tanks to ensure the production standards of food and drugs.