Whether animal cell culture technology can be industrialized and commercialized on a large scale depends on whether a suitable bioreactor can be designed. Due to the great difference between animal cells and microbial cells, traditional microbial reactors are obviously not suitable for large-scale culture of animal cells. First of all, it must be satisfied that under the condition of low shear force and good mixing, sufficient oxygen can be provided for cell growth and cell synthesis of products.
At present, bioreactors for animal cell culture mainly include: spinner flask incubator, plastic bag multiplier, packed bed reactor, multi-layer plate reactor, spiral membrane reactor, tubular spiral reactor, ceramic rectangular channel honeycomb Reactors, fluidized bed reactors, hollow fiber and other membrane reactors, stirred reactors, airlift reactors, etc.
According to the different ways of culturing cells, these reactions can be divided into the following three categories:
1. Reactors for suspension culture: such as stirred reactors, hollow fiber reactors, ceramic rectangular channel honeycomb reactors, and airlift reactors;
2. Reactor for adherent culture : such as stirring reactor (microcarrier culture), glass bead bed reactor, hollow fiber reactor, ceramic rectangular channel honeycomb reactor;
3. Reactor for embedded culture: such as flow Fluidized bed reactor, solidified bed reactor.
Culture comparison of animals, plants and microbial cells