Fermentation tanks have been widely used in the pharmaceutical, enzyme, monosodium glutamate, and food industries as a device for microbial fermentation. It improves fermentation efficiency by optimizing the fermentation conditions of microorganisms. According to the aerobic and anaerobic properties of the cultured microorganisms, fermenters can be divided into aerobic and anaerobic types. Fermentation tanks generally have good liquid mixing ability, heat transfer rate, and reliable detection and control instruments. Then the following is a detailed introduction to the basic simple structure of the fermentation tank.
1. Dissolved oxygen control system
Air flow meter, manually adjust the dissolved oxygen level in the fermentation broth by adjusting the size of the air flow. Stirring motor and stirring linkage: The stirring motor of the fermentation tank provides rotational power to drive the stirring linkage to rotate; the blades of the latter stir the fermentation liquid, break up the air bubbles, and increase the contact interface of gas and liquid, thereby increasing the dissolved oxygen level.
2. Tank
The volume of the fermenter used in the laboratory is generally several liters and tens of liters, and the tank is usually composed of glass. In order to meet the process requirements, the tank must be able to withstand the working pressure and temperature during fermentation and sterilization. The thickness of the tank wall depends on the tank diameter, material and pressure resistance.
3.Detection device: The detection devices of typical fermentation tanks are :
(1) Temperature probe: monitor the temperature change during the cultivation process;
(2) Dissolved oxygen electrode: directly immersed in the fermentation broth to monitor the dissolved oxygen change in the fermentation broth;
(3) pH electrode: directly immersed in the fermentation broth to monitor the pH changes in the fermentation broth.
4.The temperature control system
Includes the cooling water pipe at the bottom of the fermentation tank and the cooling water pipe on the condenser at the air outlet. Since heat is usually generated during the fermentation process, cooling water can be passed in to maintain a constant temperature.
5. The acid-base balance device
can pump the acid or alkaline solution into the fermentation broth through the peristaltic pump to adjust its pH value.
6. Heat-exchanger rig
During the fermentation process of the heat exchange device, the heat generated by biological oxidation must be removed to ensure that the fermentation is carried out at a constant temperature.
7. The inoculation port of other devices
Is used to insert the seed liquid into the fermenter through the inoculation port, and it can also supplement nutrients during the fermentation process. Sampling port, through which a certain amount of fermentation broth can be taken out from the fermenter for various detection and analysis.